Transformation of the white-rot basidiomycete Coriolus hirsutus using the ornithine carbamoyltransferase gene.

نویسندگان

  • Akira Tsukamoto
  • Yasushi Kojima
  • Yukio Kita
  • Jun Sugiura
چکیده

An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg+ with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 10(3)-10(4) transformants per mug DNA per 10(6)-10(7) oidial protoplasts was reached. Southern blots showed that the transforming DNA was integrated into chromosomal DNA with multi-copies. The Arg+ phenotype of the transformants was stably inherited through mitosis.

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عنوان ژورنال:
  • Bioscience, biotechnology, and biochemistry

دوره 67 10  شماره 

صفحات  -

تاریخ انتشار 2003